Vena8 Fluoro+ Biochip Tutorial
Автор: we are cellix
Загружено: 2010-11-15
Просмотров: 41138
Описание:
Vena8 Fluoro+ biochip protocol, ideal for thrombosis assays, Vena8 Fluoro+ biochip is compatible with confocal microscopy and immunostaining. Tutorial illustrates coating of biochip with adhesion molecules (e.g. VWF, collagen, fibrinogen, VCAM, ICAM etc.); conducting an assay with cell suspension (e.g. platelets, whole blood, T-cell, monocytes, neutrophils, eosinophils, PBMCs etc.) and analysis of data. Quantitate data outputs include IC50 curves of cell adhesion (e.g. platelet adhesion, aggregation, thrombi formation) vs. shear flow rate (mimicking in vivo arterial/venous/capillary flow); morphology analysis of cell adhesion and migration; tracking of flowing, rolling and migrating cells. Ideal for functional cell-based assays for cell-ligand interactions; preclniical drug development and lead optimization.
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