UV vis spectrophotometer calibration
Автор: Reesach
Загружено: 2025-07-30
Просмотров: 158
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The video provides a detailed explanation of:
Principle: The video explains that the spectrophotometer's principle is based on the Beer-Lambert Law, which states that the absorbance of a solution is directly proportional to its concentration and the path length of the light.
Calibration Parameters: It outlines the six key calibration parameters:
Control of Absorbance
Control of Wavelength
Limit of Stray Light
Resolution Power
Linearity
Matching of Cells
Calibration Process: The video describes the specific chemicals and procedures used for each calibration parameter, including the use of Potassium Dichromate, Holmium Oxide, and Toluene.
This tutorial is a helpful resource for anyone seeking to understand the fundamental principles and calibration process of a UV-Visible spectrophotometer.
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