Related Substances Calculation for HPLC and GC
Автор: Reesach
Загружено: 2025-08-15
Просмотров: 139
Описание:
This video by Advith & Kavish explains the three main calculation methods for Related Substances (RS) in High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). The three methods are:
Area Normalization: A method that calculates the percentage of a peak by dividing its area by the sum of all detected peaks. The video clarifies that a standard is not used in this calculation.
Times Calculation: This method is used when a pharmacopoeia monograph specifies a limit based on a multiple of a reference solution. It is unique because the standard is prepared by diluting the test sample itself.
Assay Calculation: This method involves a separately prepared standard with its own potency and dilution for calculating impurities.
RS
related Substances
HPLC
GC
Calculation
RS Calculation
Area normalization
area normalisation
times calculation
Impurity
Impurities
Related Reponse factor
RRF
Indian pharmacopeia
IP monograph
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#Rs
#RSHplc
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