Flourescent Proteins Expression by mRNA Injection | Protocol Preview

Описание: Watch the Full Video at https://www.jove.com/v/52042/expressi....

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes - a 2 minute Preview of the Experimental Protocol

Estelle Hirsinger, João Emanuel Carvalho, Christine Chevalier, Georges Lutfalla, Jean-François Nicolas, Nadine Peyriéras, Michael Schubert
Institut Pasteur, Département de Biologie du Développement et Cellules Souches; Sorbonne Universités, Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06); Centre de Recherche en Cancérologie de Marseille, Equipe Epigenetic Control of Normal and Pathological Hematopoiesis; CNRS UMR5235/DAA/cc107/Université Montpellier II, Unité de Dynamique des Interactions Membranaires Normales et Pathologiques; CNRS-NED, Institut de Neurobiologie Alfred Fessard, Plateforme BioEmergences IBiSA FBI;

We report here the robust and efficient expression of fluorescent proteins after mRNA injection into unfertilized oocytes of Branchiostoma lanceolatum. The development of the microinjection technique in this basal chordate will pave the way for far-reaching technical innovations in this emerging model system, including in vivo imaging and gene-specific manipulations.

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments