Cellular Senescence Induction and Validation in Primary Human cells | Protocol Preview

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Induction and Validation of Cellular Senescence in Primary Human Cells - a 2 minute Preview of the Experimental Protocol

Alejandra Hernandez-Segura, Simone Brandenburg, Marco Demaria
University of Groningen, University Medical Center Groningen, European Research Institute for the Biology of Aging;

Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.

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