Gene Expression Tracing by detecting Galactosidase Activity | Protocol Preview

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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos - a 2 minute Preview of the Experimental Protocol

María José Blanco, Ana I.R. Learte, Miguel A. Marchena, Emma Muñoz-Sáez, María Antonia Cid, Iván Rodríguez-Martín, Cristina Sánchez-Camacho
Universidad Europea de Madrid, Faculty of Biomedical and Health Sciences; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC),; Universidad Europea de Madrid, School of Doctoral Studies and Research;

Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.

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