Subcellular detections

Описание: Subcellular detections in QuPath are a convenient way of analyzing in-situ hybridization data. Sara McArdle explains how to use them for the analysis of RNAscope data.

00:00:00 Sara McArdle explains how to use subcellular detections in QuPath
00:04:26 Subcellular detections
00:03:39 Creating full image annotation and detecting cells
00:04:55 Setting up detection thresholds
00:06:16 Adjusting detection parameters
00:07:52 Splitting clusters by shape and intensity
00:09:20 Estimated number of spots
00:10:18 Running spot detection on all cells
00:10:55 Trick: use - to temporarily disable spot detection

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