Gel Filtration manual procedure with PhyNexus gel filtration columns

Описание: https://www.biotage.com/gel-filtratio...

Biotage gel filtration columns empower researchers to prepare and process large numbers of potential antibody and protein leads by delivering a fully automated desalting and buffer exchange solution. Gel filtration columns can process up to 96 samples in about 30 minutes, saving hours of time, delivering more consistent and reproducible results, and maintaining protein functionality.

Biotage gel filtration columns can be fitted to most 8, 12, and 96-channel automation liquid handling instruments equipped with gripper arm for moving tip trays. The gel filtration columns are top loaded with sample; gravity flow pulls the sample through the gel, and the automated gripper moves the tray as necessary for desalting, buffer exchange or fractionation capture.

The PhyTip column’s unique dual frit design creates the perfect conditions for a controlled, effective flow through the gel trapped between the frits.

• PhyTip gel filtration desalting buffer exchange columns operate as small scale, chromatographic separation columns.
• PhyNexus gel filtration columns are the only high throughput, automated, chromatography desalting solution.
• Similar to the PhyTip affinity columns, the resin of choice is positioned between two inert screens that maintain the resin bed at the base of the column.
• Unlike the affinity columns, the sample is passed unidirectionally through the column by gravity flow.

High Performance:
• Remove more than 95% of salts with less than 80% yield of protein
• Retain protein functionality
• Less than 10% CV for volume and concentration between final samples
• Ready for downstream assay

Automated desalting/buffer exchange of functional proteins:
• Fully automated process with MEA Personal Purification System
• 12 or 96 samples desalted/buffer exchanged in ~30 minutes.

High throughput format:
• Available in 96 sample format for high throughput processing

Product comparisons:
• Complete in a fraction of the time of dialysis – ~30min compared to overnight.
• Unlike spin columns, PhyTip columns simulate traditional chromatography; can control flow and fractionation.
• PhyNexus gel filtration columns are the only system that is reproducible, automated, and high throughput.
PhyTip gel filtration columns are compatible with most 8, 12, and 96-channel automation liquid handling robots including Agilent Technologies, Beckman Coulter, Dynamic Devices, Perkin Elmer, Hamilton, and Tecan.