How to perform high-throughput buffer exchange and desalting with PhyTip columns

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Biotage gel filtration columns empower researchers to prepare and process large numbers of potential antibody and protein leads by delivering a fully automated desalting and buffer exchange solution. Gel filtration columns can process up to 96 samples in about 30 minutes, saving hours of time, delivering more consistent and reproducible results, and maintaining protein functionality.

Biotage gel filtration (also known as size exclusion chromatography) tips are compatible with all major 8, 12, and 96-channel Hight Throughput Gel Filtration Desalting Columns automation liquid handling instruments equipped with gripper arm for moving tip trays. The gel filtration columns are top loaded with sample; gravity flow pulls the sample through the gel, and the automated gripper moves the tray as necessary for desalting, buffer exchange or fractionation capture.

Gel filtration desalting buffer exchange chromatography columns operate as small scale, chromatographic separation columns.
Biotage gel filtration chromatography columns are the only high throughput automated chromatography desalting solution.
Similar to the PhyTip affinity columns, the resin of choice is positioned between two inert screens that maintain the resin bed at the base of the column.
Unlike the affinity columns, the sample is passed unidirectionally through the column by gravity flow.
Columns packed with 200 µL and 600 µL columns of 5K or 10K gel filtration resin can accommodate sample volumes from 20 µL to 400 µL.

High Performance
Remove greater than 95% of salts with greater than 80% yield of protein
Retain protein functionality
Less than 10% CV for volume and concentration between final samples
Ready for downstream assay

Automated desalting and buffer exchange of functional proteins
Fully automated process with all major liquid handling robots with a gripper
12 or 96 samples desalted/buffer exchanged in ~30 minutes.

High throughput format
Available in 96 sample format for high throughput processing

Product comparisons
Complete in a fraction of the time of dialysis – ~15 min compared to overnight.
Unlike spin columns, PhyTip columns simulate traditional chromatography and can control flow and fractionation.
Biotage gel filtration chromatography columns are the only system that is reproducible, automated, and high throughput.