Hippurate Hydrolysis Test | Principle, Procedure & Interpretation | Systematic Bacteriology | BMLS
Автор: Shrii!!!!BMLS Pathshala
Загружено: 2026-02-11
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Hippurate Hydrolysis Test | Principle, Procedure & Interpretation | Systematic Bacteriology | BMLS
Hippurate Hydrolysis Test is an important biochemical test in Systematic Bacteriology used for the identification of bacteria like Streptococcus agalactiae and Campylobacter jejuni.
In this video, we explain the Principle, Reagents, Procedure, Result Interpretation, and Clinical Significance of the Hippurate Test in a simple and exam-oriented way for BMLS students.
This topic is highly important for BMLS 3rd/4th semester, BMLT, DMLT, and MLT practical exams & viva.
🧪 Topics Covered:
🔬 What is Hippurate Hydrolysis Test?
🧫 Principle of Hippurate Hydrolysis
🧴 Reagents Used (Sodium Hippurate & Ninhydrin)
⚗️ Step-by-Step Procedure
🎨 Result Interpretation (Positive & Negative)
🦠 Organisms Identified
📚 Viva & University Exam Points
📖 Principle (Short SEO Paragraph)
The Hippurate Hydrolysis Test is based on the ability of certain bacteria to hydrolyze sodium hippurate into benzoic acid and glycine. The released glycine reacts with ninhydrin reagent, producing a deep purple color, indicating a positive result.
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Streptococcus agalactiae identification
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BMLS microbiology
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DMLT bacteriology
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