(53) Final Paper all Units | Instrumental Methods of Analysis | B.Pharma | Chromatographic Technique

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UNIT –I
UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, the solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-
Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
Applications - Spectrophotometric titrations, Single component and multi component analysis

Fluorimetry
Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications

UNIT –II
IR spectroscopy
Introduction, fundamental modes of vibrations in polyatomic molecules, sample handling, factors affecting vibrations Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry-Principle, interferences, instrumentation and applications

Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications
Nepheloturbidometry- Principle, instrumentation and applications

UNIT –III
Introduction to chromatography: Adsorption and partition column chromatography-Methodology, advantages, disadvantages and applications.

Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.

Paper chromatography-Introduction, methodology, development techniques, advantages, disadvantages and applications

Electrophoresis–Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications

UNIT –IV
Gas chromatography - Introduction, theory, instrumentation, derivatization,temperature programming, advantages, disadvantages and applications

High-performance liquid chromatography (HPLC)-Introduction, theory,instrumentation, advantages and applications.

UNIT –V
Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications.
Gel chromatography- Introduction, theory, instrumentation and applications
Affinity chromatography- Introduction, theory, instrumentation and applications

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