Don't be fooled by imidazole! It absorbs UV280, just like protein – on chromatogram it'll be seen!
Автор: the bumbling biochemist
Загружено: 2026-02-24
Просмотров: 677
Описание:
Imidazole is great for purifying His-tagged proteins, but it's a pain for when you want to detect and measure concentration of proteins using UV280 absorbance!
More on His-tag purification: http://bit.ly/histagpurification
More on determination of protein concentration: http://bit.ly/proteinmeasuring
More on chromatograms: https://bit.ly/chromatograms
More on elution types: https://bit.ly/elution & • Elution terms, strategies (stepwise, gradi...
More on desalting & buffer exchange: https://thebumblingbiochemist.com/365... & • Techniques for buffer exchange (Desalting ...
more on protein chromatography: http://bit.ly/proteincleaning & • Protein chromatography
more practical lab tips & tricks: https://bit.ly/lab_tricks_page & • Practical lab tips & tricks
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com
#scicomm #biochemistry #molecularbiology #biology #sciencelife #science #realtimechem
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