How to Set Up Presets in EPU for Cryo-EM Data Collection

Описание: In this tutorial, we demonstrate how to set up and configure Presets in EPU for cryo-electron microscopy (cryo-EM) data collection. Presets define microscope and camera settings at different magnifications and acquisition stages, forming the foundation of an efficient and reproducible EPU workflow. Properly configured Presets are essential for smooth transitions between Atlas, Grid Square, Hole, and Data Collection modes.

This video walks through the key parameters involved in Preset configuration, including magnification, beam settings, dose, and camera setup, highlighting best practices for reliable automated data collection.

📌 This video is part of our step-by-step Cryo-EM training series using EPU.
🎥 Watch the full playlist here:    • Cryo-EM with EPU: Step-by-Step Tutorials  

For more information on Workshops & Training at PNCC: https://pncc.labworks.org/training/wo...

Apply to conduct research at our facility: https://pncc.labworks.org/access

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The Pacific Northwest Center for Cryo-EM (PNCC) is a state-of-the-art electron microscopy user facility funded by the National Institutes of Health Common Fund and operated jointly by Oregon Health & Science University and Pacific Northwest National Laboratory. Our mission is to serve researchers from a diverse range of backgrounds to tackle the most challenging scientific problems and train the next generation of cryo-electron microscopy specialists and users.

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