ycliper

Pacific Northwest CryoEM Center (PNCC)

PNCC offers users a seamless workflow from sample analysis and optimization to grid preparation, automated high-resolution micrograph collection, image analysis, 3D reconstruction and model construction and validation. Multiple levels of service and access are available.

The partnership between Oregon Health & Science University (OHSU) and Pacific Northwest National Lab (PNNL) combines the strengths of both institutions to create a robust center for Cryo-EM in the affordable, accessible, and attractive city of Portland, Oregon. PNCC is housed in a custom-built low-vibration microscopy suite in the Robertson Life Sciences Building (RLSB) along the south waterfront on S.W. Moody Avenue with adjacent parking and a public transit streetcar stop out front.

Interested in utilizing PNCC for your research? A step-by-step guide to submitting a research proposal can be found at: https://pncc.labworks.org/access

How to Set Up Presets in EPU for Cryo-EM Data Collection

Выполнение прямого выравнивания на микроскопе 200 кВ с освещением без интерференционных полос

Как собирать данные крио-ЭМ с использованием сетки Лэйси в EPU

EPU Multigrid: сбор данных из нескольких сетей за один сеанс

Oliver Clarke - Practical validation in cryoEM (and common pitfalls)

Justin Flatt - Advancing Data Management and Validation of Cryo-EM Structures at the wwPDB

Thomas Mulvaney/Aaron Sweeney - ChemEM & TEMPy-ReFF:Model building from small molecules to complexes

Dominika Borek - Practical approaches to low-resolution cryo-EM model building and validation

Ewan McRae - Challenges associated with cryo-EM structure determination and modeling of RNA

Greg Pintilie - 3DEM map-model validation Using Q-scores

Tom Goddard - Practical structure prediction with AlphaFold 3, Boltz 2 and ChimeraX

Greg Chojnowski - Structural interactomics, or how to get the most out of EM, MX, and AI

Michael Chapman - Calculating an expected EM map to optimize consistency between model & experiment

Daisuke Kihara - Structure modeling, validation, and refinement assisted by deep learning

Mark Herzik - ML approaches to cryoEM density modification differentially affect biomacromolecule...

Paul Emsley - Cryo-EM modelling with Coot and Moorhen

Pavel Afonine - Refinement and validation in Phenix

How to Create a New Atlas Session in EPU

Cryo-EM Grid Evaluation: Manual Screening with EPU

Калибровка сдвига изображения и автофункции в EPU

Energy Filter Tuning with Sherpa: Selectris-X + Falcon4i

BioContinuum GIF Tuning and K3 DED Gain Collection

[Updated] Sample Screening with SerialEM at the Pacific Northwest CryoEM Center

Data Collection with SerialEM at the Pacific Northwest CryoEM Center

Camera Setup: Total Dose, Exposure Time, and Frames - SerialEM Tutorial

Record to View Low Dose Offset - SerialEM Tutorial

Krios Autoloader Inventory - Pacific Northwest CryoEM Center

Arctica TEM User Interface Overview - Pacific Northwest CryoEM Center

Multishot Setup - SerialEM Tutorial

AAV Chimera Spin