M. Mourino + E. Rieder - Attenuated FMD vaccines (FMD-LL3B3D vaccine platform: safe, highly [...]

Описание: M. Mourino + E. Rieder - Attenuated FMD vaccines (FMD-LL3B3D vaccine platform: safe, highly potent, fully diva compatible, inactivated FMD virus vaccines)

Open Session of the EuFMD – 2018 – Increasing Global Security in the supply of effective vaccines – 29-31 October 2018 -Borgo Egnazia, Italy

Introduction
Traditional chemically-inactivated foot-and-mouth disease virus (FMDV) vaccines are used to control FMD around the world in spite of drawbacks - (1) large quantities of virulent FMDV are used, with the risk of virus escaping from manufacturing facilities or incomplete inactivation during the vaccine formulation process; (2) traditional vaccines produced from wild type FMDV are not fully compatible with a DIVA approach, since small amounts of nonstructural proteins (NSPs) may still be present; and (3) they do not fully protect animals from persistent infection.

Materials and methods
A novel, marked FMD-LL3B3D vaccine platform under development by Zoetis Inc. and The United States Department of Agriculture - Agricultural Research Service, consists of an attenuated virus platform containing negative markers in the NSPs 3B and 3Dpol. This vaccine platform allows for the easy exchange of capsid coding sequences. In contrast to wild-type FMD vaccine viruses, the FMD-LL3B3D vaccine platform viruses induce no clinical signs of FMD and no shedding of virus in cattle or pigs when inoculated as a live virus. This vaccine platform uses existing FMD vaccine manufacturing technology without the biosafety risk associated with FMD vaccine production.

Results
Cattle immunized with a variety of inactivated FMD-LL3B3D vaccine constructs formulated with a proprietary adjuvant system were protected from challenge with parental virus. Two negative markers allow the FMD-LL3B3D vaccines to be fully DIVA compatible.

Discussion
This vaccine platform, currently undergoing development, provides opportunities for safer and higher potency FMD vaccines in support of global disease control and eradication programs.

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