Webinar: Development of a Scalable Islet Cryopreservation Method that Works
Автор: VitaCyte
Загружено: 2022-09-08
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Presented by Dr. Erik Finger, University of Minnesota
The search for a method for islet cryopreservation that achieves both high viability and clinical scalability began in 1981. Numerous attempts to cryopreserve islets by slow cooling islets in cryoprotective agent (CPA) solutions have been unsuccessful. A multidisciplinary team from the University of Minnesota, including engineers and transplant biologists, solved this problem by developing a vitrification method where the islets are cryogenically stored in an ice-free glassy state. Their approach was to optimize heat transfer and minimize CPA toxicity. This method is clinically scalable. Human, pig, mouse, and human stem cell-derived islets could all be cryopreserved and demonstrated normal metabolic activity in vitro or in vivo (see https://www.nature.com/articles/s4159.... The webinar will present an overview of the method, review the results comparing cryopreserved to fresh islets, and the implications of using cryopreserved islets for research or islet transplantation.
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