SpliceWiz - easy splicing analysis and visualization in R

Описание: SpliceWiz - easy splicing analysis and visualization in R

Alex Wong (Centenary Institute, Australia)

2:30 PM - 2:45 PM AEDT (Australian Eastern Daylight Time) on Thursday, Dec. 1st, 2022
LONG TALK

Alternative splicing allows genes to express multiple mRNA isoforms with different, and sometimes opposite, biological functions. In this way, alternative splicing adds an additional dimension to gene expression which is often incompletely understood and ignored in routine RNA sequencing analysis. SpliceWiz is a R/Shiny graphical user interface (GUI) that simplifies splicing analysis and visualization from aligned RNA sequencing data. The pipeline is highly performance focused, including multi-threaded BAM file parsing; a new compression format for storing stranded sequencing coverage data; on-disk memory management using DelayedArrays; and a selection of differential analysis pipelines using downstream established statistical tools. Interactive heatmaps, scatter and volcano plots facilitate data exploration allowing users to easily discover splicing events of interest. Importantly, SpliceWiz introduces a powerful visualization technique to demonstrate differential alternative splicing differences between conditions using group-averaged sequencing coverage plots. In summary, SpliceWiz is a user-friendly and computationally powerful alternative splicing analysis and visualization tool catering for a broad range of users.

Moderator: Peter Hickey (WEHI, Australia)