How to Validate Invitrogen Antibodies for Proven Specificity

Описание: In this short video, see how Invitrogen™ antibodies undergo rigorous validation using multiple methods to ensure consistent, reliable results in every experiment: https://www.thermofisher.com/us/en/ho...

Watch More:    • Antibodies Explained: What They Are, Types...  

What You'll Learn:
• How Invitrogen™ antibodies are tested for specificity and reproducibility
• The five pillars of antibody validation used across applications
• How validation ensures performance in western blot, ICC, and flow cytometry

Materials Used:
• Invitrogen™ antibodies and validation reagents
• Western blot, immunocytochemistry (ICC), and flow cytometry platforms
• Cell and tissue samples expressing target proteins
• Detection reagents and imaging systems

Steps Covered in Video:
1. Select target-specific Invitrogen™ antibodies.
2. Validate antibody specificity using multiple complementary methods.
3. Confirm reproducibility across applications.
4. Deliver performance-verified antibodies for research confidence.

CHAPTERS:
0:00 – Introduction to Invitrogen antibody validation
0:15 – Overview of the five pillars of validation
0:40 – Ensuring reproducibility and performance
0:58 – Confidence in every antibody

FAQs
Q: What are the five pillars of validation?
A: Genetic, orthogonal, independent antibody, expression, and capture methods.
Q: Why does validation matter?
A: It ensures accuracy, reproducibility, and confidence in protein detection results.
Q: Which applications are validated?
A: Western blot, immunocytochemistry, and flow cytometry are among the key assays tested.

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