SGD On Clinical Biochemistry Viva: Benedict's Test, Protein Tests & Bedside Urinalysis | MBBS

Описание: In this interactive Biochemistry practical session for the 2025-2026 MBBS batch at Santiniketan Medical College & Hospital, we explore the diagnostic tests every future doctor needs to know.

From the chemistry of reducing sugars to critical "Point of Care Testing" (POCT) in clinical settings, this video provides a high-yield overview of urine biochemistry.

In this lecture, you will learn:

Benedict's Test Principles: Understand why it requires an alkaline medium and how cupric ions are reduced to cuprous ions [01:00].

False Positives in Benedict's: Discover which drugs and substances (like Vitamin C, Aspirin, and Uric Acid) can interfere with results [03:32].

Clinical Significance: Why Benedict's test is positive in Diabetes Mellitus but not in Diabetes Insipidus [04:39].

Pregnancy & Urinalysis: The difference between Lactose and Glucose in urine during pregnancy and why Proteinuria can be a "death sentence" if not managed [07:02].

Protein Detection Methods: A live demonstration of the Sulphosalicylic Acid Test and an explanation of the Heat Coagulation Test [09:07].

Bedside Testing (POCT): Why these are called "Point of Care Tests" or "Bedside Tests" in a hospital setting [08:15].

Key Laboratory Demonstrations:

Sulphosalicylic Acid Test: Watch the formation of white turbidity as a positive indicator for proteins in a urine sample [12:19].

Semi-Quantitative Analysis: Understanding how we estimate the concentration of substances based on color changes and turbidity.

Timestamps for Easy Navigation: [00:01] - Furanose vs. Pyranose Stability [01:00] - Benedict's Test Principle & Reduction Chemistry [02:04] - Identifying False Positives (Vitamin C, Aspirin, etc.) [04:13] - Clinical Conditions: Glycosuria & Diabetes Mellitus [05:10] - Diabetes Insipidus vs. Mellitus Explained [07:02] - Lactosuria in Pregnancy vs. Pathological Proteinuria [08:15] - Point of Care Testing (POCT) & Bedside Tests [09:07] - Introduction to Sulphosalicylic Acid Test for Proteins [10:03] - Protein Test Variants: Heat Coagulation & More [12:19] - Live Lab Demo: Positive Protein Result (White Turbidity) [14:19] - Practical Alternatives in Rural/PHC Settings


SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/Lecture
On: BIOCHEMISTRY by Dr. Karan Pal Singh
(MBBS Batch: 2025-26)
Conducted on: 05-01-26