SDS PAGE | Part 1 - Gel Preparation | Hindi
Автор: Baayo
Загружено: 2019-04-18
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This video is about SDS PAGE Gel Preparation process. SDS-PAGE is an analytical technique to separate proteins based on their molecular weight.
SDS PAGE | Part 2 - Principle: • SDS PAGE | Part 2 - Principle | Hindi
When proteins are separated by electrophoresis through a gel matrix, smaller proteins migrate faster due to less resistance from the gel matrix. Other influences on the rate of migration through the gel matrix include the structure and charge of the proteins.
In SDS-PAGE, the use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel largely eliminates the influence of the structure and charge, and proteins are separated solely based on polypeptide chain length.
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