Lac Operon Regulation

Описание: This video describes the organization and regulation of the lac operon. Regulation under conditions of high and low glucose/ lactose concentration are described.
Brief explanation:

E.coli is able to utilize lactose as an alternate energy source when glucose is not available. The genes in the lac operon function in the use of lactose. When lactose is not present in the environment, the lacI gene expresses a repressor protein that binds to the operator region and physically blocks the polymerase from transcribing the lac genes (lacZ, lacA, lacY). When lactose is present, it is cleaved to allolactose, which binds the repressor and derepresses transcription.
When glucose levels are low, cyclic AMP (cAMP) is high and bind to catabolite activator protein (CAP). CAP binds to the CAP site upstream of the lac promoter, enhancing transcription.



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