Quantitative estimation of blood glucose by GOD POD method
Автор: Bio4Med
Загружено: 2026-01-29
Просмотров: 64
Описание:
In this video, we perform a quantitative estimation of blood glucose levels using the enzymatic GOD-POD (Glucose Oxidase-Peroxidase) method. We cover the underlying biochemical principles, the reagents involved (GOD, POD, 4-aminoantipyrine, and phenol), the role of the standard glucose solution, and how to calculate the results using a colorimeter at 540 nm. This is an essential practical for MBBS and MD Biochemistry students.
Topics covered:
Principle of GOD-POD method (Trinder's Reaction)
Role of individual reagents
Preparation of the working reagent
Procedure and incubation times
Calculation using the standard formula
Clinical significance and normal reference ranges
00:00 Introduction
00:14 Method
02:07 Materials needed
02:40 Sample needed
04:45 Capillary blood vs Venous blood
07:13 Reagents needed
07:48 Principle
09:03 Procedure
10:22 Calculation
10:42 Result
10:54 Interpretation
12:40 Hyperglycemia
15:45 Hypoglycemia
17:10 Other methods
17:35 Glucometer
21:52 Glucometer vs Lab method
24:03 Possible viva questions
#MBBS #BiochemistryPractical #BloodGlucoseEstimation
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