Immunodetection: Outer Membrane Proteins By Flow Cytometry-Isolated Mitochondria l Protocol Preview

Описание: Watch the Full Video at https://www.jove.com/v/51887/immunode....

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria - a 2 minute Preview of the Experimental Protocol

Sarah Pickles, Nathalie Arbour, Christine Vande Velde
Université de Montréal, CRCHUM, Department of Biochemistry; Université de Montréal, CRCHUM, Department of Neurosciences;

Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.

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