Droplet-Based Microfluidic Approach & Microsphere-PCR Amplification l Protocol Preview

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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons - a 2 minute Preview of the Experimental Protocol

Se Hee Lee, Ho Won Lee, Da Som Kim, Hyuck Gi Kwon, Jong Hyun Lee, Yang-Hoon Kim, Ok Chan Jeong, Ji-Young Ahn
Chungbuk National University, College of Natural Sciences; Inje University, Department of Biomedical Engineering; Inje University, Institute of Digital Anti-Aging Healthcare;

This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.

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