i5k Webinar October 5, 2016: Pacific Biosciences
Автор: i5k Community
Загружено: 2016-10-12
Просмотров: 805
Описание:
Presenters: Dr. Jonas Korlach, Chief Scientific Officer and Dr. Sarah Kingan, Senior Scientist Bioinformatics
Presentation Title: High-Quality De Novo Insect Genome Assemblies using PacBio Sequencing
Summary:
PacBio Sequencing is characterized by very long sequence reads (averaging > 10,000 bases), lack of GC-bias, and high consensus accuracy. These features have allowed the method to provide a new gold standard in de novo genome assemblies, producing highly contiguous (contig N50 > 1 Mb) and accurate (> QV 50) genome assemblies. We will briefly describe the technology and then highlight the full workflow, from sample preparation through sequencing to data analysis, on examples of insect genome assemblies, and illustrate the difference these high-quality genomes represent with regard to biological insights, compared to fragmented draft assemblies generated by short-read sequencing.
For more information about Pacific Biosciences: http://www.pacb.com/
For more information about the i5k Webinar series: http://i5k.github.io/webinar
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