Illumina Single-Cell RNA Prep: Full Workflow & Data Analysis Guide

Описание: Join the University of Chicago SCIC (scic.pme.uchicago.edu) for an in-depth technical workshop on the Illumina Single Cell 3’ RNA Prep workflow (powered by PIPseq™ technology). This session covers everything from initial experimental design to secondary data analysis using the DRAGEN pipeline.
In this video, you will learn:
1. Workflow Overview: How Particle-templated Instant Partitions (PIPs) eliminate the need for microfluidics.
2. Experimental Design: How to scale from 2,000 to 100,000 cells and manage sample multiplexing.
3. Sample Prep & QC: Best practices for cell viability and nuclei suspension.
4. Data Analysis: A deep dive into the DRAGEN Single Cell RNA workflow and tertiary analysis options like Partek Flow.

Speakers:
Cha'Tonya Brown, PhD – Field Application Scientist (FAS), Illumina
Kelly Kyle – Bioinformatics Specialist, Illumina

Hosted by: The Single Cell Immunophenotyping Core (SCIC) at PME, the University of Chicago.

Timestamps:
0:00 Introduction to Single-Cell Technology
10:15 The PIPseq Workflow & Equipment
25:30 Experimental Design & Scaling
40:00 Sample Preparation & QC Best Practices
55:20 Data Analysis: DRAGEN Pipeline Overview
1:10:00 Tertiary Analysis & Visualization

For more information or to access these services at the SCIC, contact Fatih Abasiyanik ([email protected]).