7. Proximity dependent enzymatic biotinylation by Rosario Durán

Описание: ICGEB course “Unveiling biological networks by cutting-edge proteomics tools”, 30 November – 4 December 2021, Rosario, Argentina. (@Ros_Course).

https://www.icgeb.org/proteomics-cour...

Proximity-dependent biotinylation approaches coupled with mass
spectrometry analysis offer new ways to study protein-protein interactions
and subcellular proteomes in the context of the living cell. The presentation
included the following topics: Description of the Proximity dependent
biotinylation strategy. Main enzymes used: biotin ligases and ascorbate
peroxidases. APEX - BioID comparison. Important consideration when
designing a proximity biotinylation experiment. Some examples, including
context dependent proximity biotinylation and iBioID.

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Supported by the International Centre for Genetic Engineering and Biotechnology and organized by Germán Rosano (@GermanRosano) and Nico Blanco (@NicoEBlanco), the course focused on the latest advances in proteomic strategies, especially in the area of protein-protein interactions and proteome characterization. Lectures covered novel techniques in proteomics, and experimental sessions focused on sample preparation, mass spectrometer operation, and data analysis.