Ask me anything with Parag Mallick, Andreas Huhmer, & featuring special guest Don Kirkpatrick, Ph.D.

Описание: On this episode of Translating Proteomics, Parag, Andreas, and special guest Don Kirkpatrick answer questions submitted by the Translating Proteomics community. They cover:

• Needs in plasma proteomics
• How proteomics impacts drug development – with special guest Don Kirkpatrick, Ph.D.!
• How lifestyle impacts the proteome
• How the Nautilus Proteome Analysis Platform is impacting tau and Alzheimer’s disease research

Chapters
00:00 – Intro
01:22 – Question 1 – What are we missing in plasma proteomics and do we need absolute plasma protein abundance?
10:11 – Question 2 – Can you provide examples of proteomics driving key clinical insights or drug development efforts?
26:31 – Question 3 – How does lifestyle impact the proteome?
34:44 – Question 4 – How does Nautilus’ technology create a unique breakthrough in terms of tau and Alzheimer’s disease? Parag answers additional questions about the platform in this video:    • Parag answers questions about the Nautilus...  
45:16 – Outro

References
Shome et al., 2022 - Serum autoantibodyome reveals that healthy individuals share common autoantibodies
https://www.sciencedirect.com/science...
LaBaer Lab paper investigating autoantibody levels in plasma and their relationship to health.

Sylman et al., 2018 - A Temporal Examination of Platelet Counts as a Predictor of Prognosis in Lung, Prostate, and Colon Cancer Patients
https://www.nature.com/articles/s4159...
Mallick lab paper investigating temporal changes in platelets and their associations with cancer biology.

Krönke et al., 2014 - Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells
https://www.science.org/doi/10.1126/s...
Seminal paper describing selective protein degradation caused by lenalidomide.

Fink and Ebert 2015 - The novel mechanism of lenalidomide activity
https://ashpublications.org/blood/art...
Review of research elucidating the mechanisms of lenalidomide activity

Ndoja et al., 2025 - COP1 Deficiency in BRAFV600E Melanomas Confers Resistance to Inhibitors of the MAPK Pathway
https://www.mdpi.com/2073-4409/14/13/975
Describe links between kinase inhibitor vemurafenib and changes in ETV transcription factor degradation

Song et al., 2022 - RTK-Dependent Inducible Degradation of Mutant PI3Kα Drives GDC-0077 (Inavolisib) Efficacy
https://aacrjournals.org/cancerdiscov...
Use proteomics to discover that inavolisib acts through selective degradation of mutant PI3Kα

Canon et al., 2019 - The clinical KRAS(G12C) inhibitor AMG 510 drives anti-tumour immunity
https://www.nature.com/articles/s4158...
Covers the development of an inhibitor of KRAS mutant KRAS (G12C).

Schneider et al., 2024 - Feeding gut microbes to nourish the brain: unravelling the diet-microbiota-gut-brain axis
https://www.nature.com/articles/s4225...
Review on the gut-brain axis

Webpage for Johanna Lampe’s Lab at Fred Hutch Cancer Center
https://www.fredhutch.org/en/faculty-...

Guseh et al., 2020 - An expanded repertoire of intensity-dependent exercise-responsive plasma proteins tied to loci of human disease risk
https://www.nature.com/articles/s4159...
Work investigating the relationship between exercise, the plasma proteome, and health

Human Proteome Organization (HUPO) webpage with resources focused on proteomics in space
https://hupo.org/Human-Proteomics-at-...

Joly et al., 2025 - Large-scale single-molecule analysis of tau proteoforms
https://doi.org/10.1101/2025.06.26.66...
Nautilus preprint using Iterative Mapping of proteoforms to measure tau proteoforms in Alzheimer’s disease and related dementias samples.