Unlocking the potential of spatial biology with the MACSima™ Platform

Описание: For more information, visit https://www.miltenyibiotec.com/produc... There is a wealth of information in every tissue sample – just waiting to be unlocked. The MACSima Platform is an easy-to-use, fully automated spatial biology solution that bridges the gap between cell phenotyping and tissue imaging. It provides detailed data on the expression levels of hundreds of protein markers within the spatial framework of tissues.
Watch the video to learn how the MACSima System can stain hundreds of markers per sample for real-time sequential analysis or later composite work, while leaving the precious sample material viable for further downstream applications.
The MACSima Platform uses the principle of iterative staining with any of our 2000+ pre-tested fluorochrome-conjugated antibodies to acquire microscopy data. The process comprises three main steps, all of which are conducted in a fully automated manner.
1. Samples are stained with recombinantly engineered REAfinity™ and REAdye_lease™ antibodies from our ever-growing portfolio.
2. Immunofluorescence images of the desired sample areas are acquired.
3. The fluorescence signal is erased, either by photobleaching or by specific and controlled fluorochrome release using our sophisticated REAdye_lease antibodies. Both mechanisms can be used in the same experiment without harming sample integrity.
The process then repeats through as many iterations as you have antibodies to stain with.
While the instrument runs, users can engage in other lab activities or analyze images in real-time. The powerful MACS® iQ View Analysis Software allows for the selection and direct localization of cells based on the expression of markers. It easily handles such high volumes of data, making it simple to perform segmentation, gate cells, and scatter plots, t-SNE plots, heatmaps and much more. It’s up to the user to choose what to analyze and how to display it.
The complete platform, including the instrument, pre-tested recombinant antibodies for guaranteed reproducibility, click-in-place sample carriers and intuitive execution and analysis software empowers you to unlock new insights and accelerate your research in spatial biology.

Miltenyi Biotec is a global provider of products and services that empower biomedical discovery and advance cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies offer solutions for cellular research, cell therapy, and cell manufacturing. Our more than 30 years of expertise spans research areas including immunology, stem cell biology, neuroscience, cancer, hematology, and graft engineering. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 4,700 employees in 23 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.