Next generation CRISPR screens based on single-cell transcriptomics | Tilmann Buerckstuemmer
Автор: Twist Bioscience
Загружено: 2020-04-22
Просмотров: 6010
Описание:
We hosted Dr. Tilmann Buerckstuemmer, CSO of Aelian Biotechnology, who’ll be talking to us about leveraging two transformative technologies - CRISPR screening and single cell transcriptomics - to enable genetic screening for complex phenotypes.
During this webinar Dr. Buerckstuemmer will discuss:
how Aelian Biotechnology is using CROP-seq to expand beyond more simplistic screening read-outs.
how this screening approach can be used to map known signalling pathways and assess the impact of candidate regulators on transcriptional outcomes.
the feasibility of using both unbiased and targeted single-cell RNA sequencing and how to compare these approaches side-by-side.
the importance of Oligo Pool uniformity to ensure screening success and how the quality of Twist Oligo Pools uniquely enables these types of studies.
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