Jonas Korlach at PAG 33: Unlocking true biology with HiFi

Описание: In this opening address from PAG 33, discover how PacBio HiFi sequencing moves beyond the genome blueprint to reveal true biology through multi-omics.

PacBio Co-Founder Jonas Korlach argues that gene expression counts are insufficient metrics for understanding function and demonstrates why resolving full-length isoforms is critical. He highlights how HiFi sequencing captures the complexity of transcriptomes and mutational processes across the tree of life, contrasting these insights with the limitations of short-read RNA-seq.

What you’ll learn
Evaluate why gene expression counts are insufficient for understanding biological function.
Examine evidence showing how short-read RNA-seq fails to capture alternative splicing complexity.
Explore recent discoveries in zebrafish and the Darwin Tree of Life project enabled by HiFi data.

Featured speakers
Jonas Korlach, Co-Founder & Scientific Advisor (PacBio)

00:00 Opening
01:36 Welcome to PAG 33
02:32 From blueprint to true biology
04:06 Why isoforms matter
05:50 The short-read problem
06:20 Iso-Seq discoveries
08:17 Scaling to populations
09:05 Darwin Tree of Life study
10:18 Workshop preview

How is isoform resolution changing your understanding of your organism? Comment below.

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