DNA Sequencing: Sanger Method and Beyond Explained

Описание: In this video, I look at DNA sequencing, starting with the widely used Sanger or chain-terminating dideoxynucleotide method.

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If you're familiar with PCR, you'll grasp how this method works as I break down the process into two stages: the reaction and the separation. I look at the role of dideoxynucleotides (ddNTPs) in the reaction, how they terminate DNA chains with fluorescent markers, and how the sequence is ultimately determined.

I also look at the limitations of the Sanger method and how advanced techniques like next-generation sequencing (NGS) and third-generation sequencing are revolutionising DNA analysis, making large-scale projects and rapid sequencing possible.

Resources:
🌎 - A help sheet defining key terms is also available in the description - https://cell.drnickmorris.com/dna_seq...
📗 - The Biosciences Glossary available on Google Play Book Store - https://play.google.com/store/books/d...
📗 - Biochemistry (Stryer) - https://amzn.to/3XJ7gTR (affiliate link)
📗 - Principles of Biochemistry (Lehninger) - https://amzn.to/3ZHDVf2 (affiliate link)
📗 - Molecular Biology of the Cell (Alberts) - https://amzn.to/3zDyhQu (affiliate link)
📗 - Molecular Cell Biology (Lodish) - https://amzn.to/3zyIJZH (affiliate link)

🫖 - If you would like to say thanks for the video, then please feel free to Buy me a coffee - https://www.buymeacoffee.com/drnickm

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