Immune cell-mediated cytotoxicity: Single-cell insights using droplets and image cytometry

Описание: Traditional cell–cell interaction assays, including immune cell-mediated cytotoxicity assays, are typically performed in bulk, masking the true heterogeneity within cell populations. Samplix’s Xdrop® revolutionizes cell–cell interaction assays by enabling analysis at the single-cell level, thereby revealing population heterogeneity.

Xdrop’s single-cell encapsulation technology enables functional analysis of single cells or single cell pairs as they are isolated into 100 pL double-emulsion droplets (DE50 droplets). Acting as micro-scale incubators, these droplets allow exchange of CO2 and O2 while retaining larger secreted molecules. Consequently, single-cell droplet encapsulation not only permits the analysis of single cells but also all secreted molecules.

Moreover, Xdrop® facilitates the simultaneous analysis of multiple cellular functionalities at the single-cell level. An example is the combined cell-killing and granzyme B secretion assay, which provides mechanistic insights into the cell-killing process.

In this webinar, the Xcyto®5 imaging cytometer (Chemometec) is used to simultaneously analyze cell killing and granzyme B secretion at the single-cell level. This approach provides detailed mechanistic insights into cell-mediated toxicity. By leveraging the high-resolution capabilities of the Xcyto®5 image cytometer in combination with Xdrop’s single-cell encapsulation technology, the precise analyses of immune cell functional heterogeneity can be achieved.