Nanofluidic Junctions Creation in PDMS Microfluidic Chip | Protocol Preview

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Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles - a 2 minute Preview of the Experimental Protocol

Xi Wei, Abeer Syed, Pan Mao, Jongyoon Han, Yong-Ak Song
New York University Abu Dhabi (NYUAD), Division of Engineering; New York University Tandon School of Engineering, Department of Chemical and Biomolecular Engineering; Newomics, Inc.,; MIT, Department of Electrical Engineering and Computer Science, Department of Biological Engineering;

We propose a simple self-assembly technique of silica colloidal nanoparticles to create a nanofluidic junction between two microchannels in polydimethylsiloxane (PDMS). Using this technique, a nanoporous bead membrane with a pore size down to ~45 nm was built inside a microchannel and applied to electrokinetic preconcentration of DNA samples.

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