GEL FILTERATION CHROMATOGRAPHY

Описание: GEL FILTERATION CHROMATOGRAPHY

Gel filtration chromatography, also known as size-exclusion chromatography, is a separation technique in which molecules are separated based on their size and molecular weight as they pass through a column packed with porous gel beads (such as Sephadex, Sepharose, or Superdex). Larger molecules cannot enter the pores of the beads and therefore move faster through the column, eluting first, while smaller molecules enter the pores and take a longer, more convoluted path, eluting later. This method is widely used for purifying proteins, polysaccharides, nucleic acids, and for determining molecular weight of unknown biomolecules. It is a gentle, non-denaturing technique that preserves the biological activity of sensitive molecules, making it highly valuable in biochemical and biotechnological applications.

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