Baculovirus expression Vectors: AcMNPV
Автор: Dr. PREM-PRIMER Biotech Lectures
Загружено: 2022-10-08
Просмотров: 1757
Описание:
The specific baculovirus that has been used extensively is Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) • AcMNPV: Autographa californica Multiple N...
The production of recombinant baculovirus expression vectors involves the following steps
1. A gene of interest is inserted into the MCS of Transfer vector and propagated in E. coli.
2. Next, insect cells in culture are cotransfected with AcMNPV DNA and the Transfer vector carrying the cloned gene.
3. Selection of recombinant AcMNPV genome virus particle
Повторяем попытку...
Доступные форматы для скачивания:
Скачать видео
-
Информация по загрузке: