High Yield Culture of Neurons from Skin Fibroblasts | Protocol Preview

Описание: Watch the Full Video at https://www.jove.com/v/56904/simple-g....

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts - a 2 minute Preview of the Experimental Protocol

Shelby Shrigley, Karolina Pircs, Roger A. Barker, Malin Parmar, Janelle Drouin-Ouellet
Lund University, Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center; University of Cambridge, John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute;

Direct neuronal reprogramming generates neurons that maintain the age of the starting somatic cell. Here, we describe a single vector-based method to generate induced neurons from dermal fibroblasts obtained from adult human donors.

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