(EMSA): Study of RNA-Protein Interactions: The IRE/IRP Example

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Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example - a 2 minute Preview of the Experimental Protocol

Carine Fillebeen, Nicole Wilkinson, Kostas Pantopoulos
Jewish General Hospital, Lady Davis Institute for Medical Research; McGill University, Department of Medicine;

Here we present a protocol to analyze RNA/protein interactions. The electrophoretic mobility shift assay (EMSA) is based on the differential migration of RNA/protein complexes and free RNA during native gel electrophoresis. By using a radiolabeled RNA probe, RNA/protein complexes can be visualized by autoradiography.

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