Industrial Media Optimization: Flux, Scale, and Product Centricity

Описание: Modern #industrial fermentation treats media optimization as a dynamic control strategy rather than a static recipe to maximize metabolic flux and product quality. This approach integrates strain-centric design with advanced computational modeling to manage nutrient uptake, redox balance, and stress responses under large-scale manufacturing constraints. By precisely tuning carbon-to-nitrogen ratios and micronutrient levels, engineers can minimize metabolic overflow and ensure phenotype stability across varying #environmental conditions. Furthermore, the discussion emphasize aligning media composition with regulatory standards and downstream processing requirements to enhance economic and environmental sustainability. Ultimately, these strategies transform raw material inputs into precise levers for directing cellular resources toward high-yield, consistent industrial production.

#Bioprocess #ScaleUp and #TechTransfer,
#Industrial #Microbiology,
#MetabolicEngineering and #SystemsBiology,
#Bioprocessing,
#MicrobialFermentation,
#Bio-manufacturing,
#Industrial #Biotechnology,
#Fermentation Engineering,
#ProcessDevelopment,
#Microbiology, #Biochemistry
#Biochemical Engineering,
#Applied #MicrobialPhysiology,
#Microbial #ProcessEngineering,
#Upstream #BioprocessDevelopment,
#Downstream Processing and #Purification,
#CellCulture and #MicrobialSystems Engineering,
#Bioreaction #Enzymes
#Biocatalyst
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Timestamp
Timestamp Problem Addressed
01:50 – 02:58 Microbial "Greed" (Crabtree Effect)
03:04 – 04:03 Acetate Toxicity in E. coli
04:14 – 04:46 Redox Imbalance (NADH/NAD+)
05:01 – 05:49 Growth vs. Production Decoupling
07:13 – 07:47 Metal Precipitation at High pH
09:17 – 09:54 Antibody Glycosylation Failure
10:36 – 11:49 Scale-up Heterogeneity (Feast/Famine)
12:02 – 12:42 Phenotype Instability