His-tag protein purification with IMAC (Immobilized Metal Affinity Chromatography)
Автор: the bumbling biochemist
Загружено: 2021-07-07
Просмотров: 14312
Описание:
If you stick a bunch of histidines in a row (about 6 or 8 or so) and over a nickel column flow, that “His tag” will stick so your tagged protein you can “pick,” then compete it off with imidazole - it’s quite a cool trick! IMAC isn’t some new product release from Apple. But it is, at least for biochemists, even more exciting! IMAC stands for Immobilized Metal Affinity Chromatography and it’s a way to help specifically purify “His-tagged” proteins.
Histidine is an amino acid - it’s a normal protein letter that can link up to the other amino acids to form long polypeptide chains that fold up into functional proteins. It may be normal but it’s also special because it has this property called resonance that I’ll get into which allows it to bind metals like nickel (Ni). If you put a bunch of His in a row, they can team up to really clamp on. So if that Ni is also bound to little beads called resin you can get a His-tagged thing to stick to the resin. We often use this technique to purify proteins which we engineer to have a His tag at the end. ⠀
for text details and figures, see accompanying blog post: https://bit.ly/histagIMAC
Повторяем попытку...
Доступные форматы для скачивания:
Скачать видео
-
Информация по загрузке:
![What is a Plasma? Is it the 4th State of Matter? - [5]](https://imager.clipsaver.ru/WVYJQucn2yU/max.jpg)
