RNA Secondary Structure Prediction By High-Throughput SHAPE l Protocol Preview

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RNA Secondary Structure Prediction Using High-throughput SHAPE - a 2 minute Preview of the Experimental Protocol

Sabrina Lusvarghi, Joanna Sztuba-Solinska, Katarzyna J. Purzycka, Jason W. Rausch, Stuart F.J. Le Grice
Frederick National Laboratory for Cancer Research, RT Biochemistry Section, HIV Drug Resistance Program;

High-throughput selective 2' hydroxyl acylation analyzed by primer extension (SHAPE) utilizes a novel chemical probing technology, reverse transcription, capillary electrophoresis and secondary structure prediction software to determine the structures of RNAs from several hundred to several thousand nucleotides at single nucleotide resolution.

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