HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Описание: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
High-Performance Liquid Chromatography (HPLC) is an advanced analytical technique used to separate, identify, and quantify components in a mixture by passing a liquid mobile phase through a column packed with a fine-particle stationary phase under high pressure. As the sample travels through the column, different molecules interact variably with the stationary phase based on their polarity, size, or chemical properties, causing them to elute at different times (retention times). These separated components are then detected—commonly using UV-Visible detectors—to generate a chromatogram that shows both qualitative and quantitative information. HPLC is widely used in pharmaceuticals, biochemistry, food analysis, environmental monitoring, and clinical research due to its high accuracy, sensitivity, reproducibility, and ability to analyze complex samples rapidly.

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