Extraction Of High Molecular Weight DNA From Microbial Mats l Protocol Preview

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Extraction of High Molecular Weight DNA from Microbial Mats - a 2 minute Preview of the Experimental Protocol

Benjamin S. Bey, Erin B. Fichot, R. Sean Norman
Arnold School of Public Health, University of South Carolina, Department of Environmental Health Sciences;

We provide an improved protocol for extracting high molecular weight DNA from hypersaline microbial mats. Microbial cells are separated from the mat matrix prior to DNA extraction and purification. This enhances the concentrations, quality, and size of the DNA. The protocol may be used for other refractory samples.

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