WEBINAR — Expert Coffee Chats — Multiplex Immunoassays in Vaccine Research and Development

Описание: Join us for a chat with Bio-Rad application scientists about the current issues most relevant to your research. Jump to specific topics by following the links below.

In this episode, we discuss:
Multiplex Immunoassays in Vaccine Research and Development
Get the most data out of your sample, without spending time on individual ELISAs
Learn how multiplex assays are being used on the front lines to understand patient immune response during infection
Discuss the most commonly asked questions around targets, panels, and multi-plate data analysis

We had so many great audience questions. Watch the whole video or quickly jump to a specific question:

00:10 Introduction
3:27 What are multiplex immunoassays? And why are they helpful for vaccine research?
7:19 Can multiplex immunoassays be used to look at patients exposed to coronavirus?
10:28 What sort of adjuvants are being used for COVID-19 vaccines? Are new ones being developed? Can multiplex immunoassays be used to help test which adjuvant is best?
13:42 Regarding antibody specificity and the ability to bind to a specific target or its fragment, what are the differences between an ELISA and a multiplex assay?
16:24 What ways are vaccines evaluated to ensure they are safe and nontoxic?
20:22 Will we need to optimize the assay to run patients’ samples infected with COVID19? What are your recommendations?
23:24 I use these assays in preclinical assays, can I use them in clinical trials too?
25:00 What types of positive and negative controls are essential to obtain when developing an in-house MIA (microsphere-based immunoassay)?
27:09 Are multiplex immunoassays used in QC'ing of manufactured products? What is the benefit of ELISAs?
29:22 Can someone use the plate more than once, for example, divide the plate twice?
33:00 What type of samples do you recommend to run with Bio-Plex assays? Do you think that serum would be a good choice given that most of the COVID-19 assays start with nasal swabs?
34:25 One of the challenges in MIA for serology purpose is finding that sample high enough for all the Ab of interest. What is the highest multiplex assay for antibodies screening have you ever developed? How did you look for a reference standard curve sample? How did you test the cross-reactivity?
39:48 Do various antibody affinities (attached to beads) affect the overall outcome?
41:00 Can you describe the assay to test for various antibody isotypes after COVID disease. Antigen (spike protein) coated to bead, add patient sample, then add beads coated with antibody to detect various isotypes? Is this a kit?
43:25 How do I compare sample data collected across different days? What controls can I use?
47:21 Is throughput an issue? What about data management?
49:22 Could you elaborate a little on the choice of clonality of Ab for selection for capture and detection? What are the trade-offs of using a polyclonal ab for capture?
52:53 Has anyone used these assays in their vaccine development?
55:54 Closing

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