How to section whole brain hemispheres with a vibratome

Описание: IMPORTANT UPDATE 7-29-2025: If you are having difficulty in getting good-quality tissue sections while using a vibratome, I recommend seeing my newer video here:    • Vibratome Troubleshooting - Getting better...   .
In particular, I strongly recommend reading my pinned comment on that video, about how your choice of blade type can make a significant impact on tissue quality / consistency.

You can find the text protocol that accompanies this video on my website: https://sites.google.com/view/kru-neu...

This video gives a demonstration for sectioning rat (or mouse) brain hemispheres in an orderly and expedient way. I've also included a decent amount of discussion on small tips and rationale behind these methods. Captions are provided via auto-transcribing; they won't be perfect but hopefully they are helpful.

Thanks to Sabin Yang and Alex Camarillo for helping with filming.

Click to skip to these points:
0:00 - Intro
0:43 - Checklist of tasks to do
2:48 - Explanation of hemisecting
13:28 - Video of hemisecting (method 1)
17:28 - Video of hemisecting (method 2)
21:09 - Videos of the embedding process
26:12 - Video of gluing brain chunks onto vibratome chuck
28:12 - Video of vibratome setup
33:58 - Explanation of sectioning without solution
39:25 - Explanation of series sectioning
46:48 - Video of series sectioning
48:27 - Storing unfinished, embedded tissue