Single Virion Atomic Force Microscopy & Super-Resolution Fluorescence Imaging l Protocol Preview

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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging - a 2 minute Preview of the Experimental Protocol

Jeffery A Hodges, Saveez Saffarian
University of Utah, Department of Physics & Astronomy; University of Utah, Center for Cell and Genome;

The attachment of virions to a surface is a requirement for single virion imaging by Super-resolution fluorescence imaging or atomic force microscopy (AFM). Here we demonstrate a sample preparation method for controlled adhesion of virions to glass surfaces suitable for use in AFM and super-resolution fluorescence imaging.

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