Correlative AFM and STED – Rupture of microtubules in real-time
Автор: Bruker JPK BioAFM
Загружено: 2018-04-06
Просмотров: 403
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The video demonstrates a rupture of selected microtubules within a living human skin fibroblast cell. The microtubules are manipulated with the JPK NanoWizard® Atomic Force Microscope (AFM) and imaged with Stimulated Emission Depletion (STED) microscopy. The microtubules were labelled with SiR Tubulin and imaged with an Abberior Instruments Expert Line STED microscope (excitation, 640 nm; STED, 775 nm). The AFM manipulation path is marked with an arrow, the manipulation force was set to 5 nN with a velocity of 1 µm/s. The bending, manoeuvring and rupturing of the microtubules by the AFM tip can clearly be monitored by the STED microscope in real-time. Microtubules can be targeted and manipulated on the nanometer scale via the JPK DirectOverlay™ feature. It is also possible to adapt the manipulation force to stretch specific microtubules. This can provide more information about the elastic behaviour of microtubules.
Sample courtesy: Abberior Instruments GmbH
Read more about JPK’s AFM systems and their applications for the materials, life & nano sciences on our website: https://www.jpk.com/products/atomic-f...
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