Spirochaete Staining by Simple Negative Stain
Автор: DBT VNSGU
Загружено: 2022-01-17
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Spirochaete Staining by Negative Staining Method
• Take a clean glass slide.
• Place a small drop of Nigrosine at the end of the slide.
• With the help of toothpick gently scrape out tartar from interspaces between teeth.
• Mix this tooth tartar into the drop of Nigrosine with the help of toothpick.
• Do not spread the drop or let dry.
• Using the end edge of another slide, spread the drop out to produce a smear varying from bluish black to gray.
• Do not heat fix the slide.
• Let the smear air dry.
• Examine under oil immersion lens (100X) of compound microscope.
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