How to Isolate PBMCs from Whole Blood Using Ficoll (+ Troubleshooting Common Issues)
Автор: Log's Lab
Загружено: 2024-11-03
Просмотров: 1686
Описание:
In this video I'll show step-by-step how I isolate peripheral blood mononuclear cells (PBMCs) from whole blood using density gradient centrifugation. This should work with Ficoll-Paque™ or Lymphoprep™. I'll also go over common errors or issues, and how you can avoid or fix them.
I'll first go over materials needed, the protocol of layering blood over the density medium to isolate PBMCs, color differences, RBC contamination, and staining issues. Thanks for watching the first video on this channel!
I don't have the written protocol right now, I'll update that in a day or so.
Timestamps below.
00:00 - Intro
2:09 - Materials
4:40 - Ficoll Protocol
12:47 - Breaking the Layer
13:57 - Why the Layer is Important
15:09 - Color Variations
16:08 - Fixing RBC Contamination
17:57 - Trypan Blue Issues
18:31 - Outro
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