ONE-STAGE ASSAY OF FACTOR VIII (FVIII) – LAB MANUAL: VIDEO 6

Описание: DIAGNOSIS OF HEMOPHILIA AND OTHER BLEEDING DISORDERS
A LABORATORY MANUAL

6. IMPORTANCE OF STRAIGHT AND PARALLEL LINES FOR IQC AND REFERENCE PLASMAS

00:00:15 - Importance of straight and parallel lines for IQC and reference plasmas
00:01:28 - Calculating the concentration of FVIII in the IQC sample
00:03:24 - Calculating corrected FVIII concentration in the IQC sample
00:05:12 - Evaluating obtained IQC values, versus the expected/target concentration range
00:05:45 - Plotting results for Patient #1
00:06:50 - Calculating concentration of FVIII in Patient sample #1
00:07:32 - Calculating corrected concentration of FVIII in Patient sample #1
00:07:45 - Interpreting result for Patient #1
00:07:57 - Plotting results for Patient #2
00:08:38 - Example of results seen in presence of lupus anticoagulant
00:09:53 - Conditions for estimating FVIII concentration despite interference from lupus anticoagulant
00:12:07 - Importance of avoiding assaying just one dilution of a patient sample
00:13:16 - Plotting results for Patient #3
00:14:27 - Interpreting results for Patient #2: very similar clotting times for all three dilutions, and very similar to blank
00:14:53 - It's highly likely this patient has no FVIII
00:16:09 - Causes of nonparallelism between patient sample and standard lines
00:17:22 - Correcting for different dilutions